Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide

Kenji Osumi; Yoshitaka Makino; Eri Akaike; Takashi Yamanoi; Mamoru Mizuno; Midori Noguchi; Toshiyuki Inazu; Kenji Yamamoto; Kiyotaka Fujita

doi:10.1016/j.carres.2004.08.014

Mucor hiemalis endo-beta-N-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide

Carbohydr Res. 2004 Oct 20;339(15):2633-5. doi: 10.1016/j.carres.2004.08.014.

Authors

Kenji Osumi¹, Yoshitaka Makino, Eri Akaike, Takashi Yamanoi, Mamoru Mizuno, Midori Noguchi, Toshiyuki Inazu, Kenji Yamamoto, Kiyotaka Fujita

Affiliation

¹ The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan.

PMID: 15476727
DOI: 10.1016/j.carres.2004.08.014

Abstract

We found that the recombinant endo-beta-N-acetylglucosaminidase of Mucor hiemalis (Endo-M) expressed in Candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside) in a good yield of 43%.

MeSH terms

Acetylglucosamine / analogs & derivatives*
Acetylglucosamine / metabolism
Acetylglucosaminidase / metabolism*
Cloning, Molecular
Glycopeptides / metabolism*
Glycosylation
Mucor / enzymology*
Oligosaccharides / metabolism*
Ovalbumin
Recombinant Proteins

Substances

Glycopeptides
Oligosaccharides
Recombinant Proteins
4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside
Ovalbumin
Acetylglucosaminidase
Acetylglucosamine