Abstract
Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Typing Techniques / methods*
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Campylobacter / classification*
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Campylobacter / genetics*
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Campylobacter / isolation & purification
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Campylobacter Infections / epidemiology
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Campylobacter Infections / microbiology
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Cattle
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Disease Outbreaks
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Electrophoresis, Gel, Pulsed-Field
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Flagellin / genetics*
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Genotype
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Humans
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Sequence Analysis, DNA*
Substances
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Flagellin
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flaA protein, bacteria
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flaB flagellin