Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations

J Clin Microbiol. 2004 Oct;42(10):4840-2. doi: 10.1128/JCM.42.10.4840-4842.2004.

Abstract

Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / methods*
  • Campylobacter / classification*
  • Campylobacter / genetics*
  • Campylobacter / isolation & purification
  • Campylobacter Infections / epidemiology
  • Campylobacter Infections / microbiology
  • Cattle
  • Disease Outbreaks
  • Electrophoresis, Gel, Pulsed-Field
  • Flagellin / genetics*
  • Genotype
  • Humans
  • Sequence Analysis, DNA*

Substances

  • Flagellin
  • flaA protein, bacteria
  • flaB flagellin