Solid support membranes for ion channel arrays and sensors: application to rapid screening of pharmacological compounds

Biochim Biophys Acta. 2004 Oct 11;1665(1-2):184-90. doi: 10.1016/j.bbamem.2004.07.010.

Abstract

The use of solid supported membranes (SSM) was investigated for reconstitution of ion channels and for potential application to screen pharmacological reagents affecting ion channel function. The voltage-gated Kv1.5 K+ channel was reconstituted on an SSM and a current was measured. This current was dependent on the presence of K+, but not Na+, indicating that the Kv1.5 K+ channel maintained cation specificity when reconstituted on SSM. Two pharmacological reagents applied to Kv1.5 K+ channels reconstituted on SSM had similar inhibitory effects as those measured using Kv1.5 in biological membranes. SSM-mounted ion channels were stable enough to be washed with buffer solution and reused many times, allowing solution exchange essential for pharmacological drug screening.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Drug Evaluation, Preclinical / methods*
  • Equipment Reuse
  • Ion Channels / drug effects*
  • Kv1.5 Potassium Channel
  • Membranes, Artificial*
  • Mice
  • Patch-Clamp Techniques
  • Potassium Channels, Voltage-Gated / drug effects

Substances

  • Ion Channels
  • Kcna5 protein, mouse
  • Kv1.5 Potassium Channel
  • Membranes, Artificial
  • Potassium Channels, Voltage-Gated