Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE. PCR primers were designed according to the three Wx genes sequences and their mutants, respectively. A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus, respectively, corresponding to the normal and mutant alleles. Compared with the former studies, shorter and more different PCR products at three loci, amplified by the primers designed for Wx-B1 gene can be separated in 2% agarose gel, which enables screening breeding lines for noodle use faster and effectively.