Abstract
The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NV strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Caliciviridae Infections / transmission
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Caliciviridae Infections / virology
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DNA, Viral / genetics
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Feces / virology
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Food Microbiology
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Gastroenteritis / virology
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Humans
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Norovirus / genetics*
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Norovirus / isolation & purification*
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Norovirus / pathogenicity
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Reverse Transcriptase Polymerase Chain Reaction / statistics & numerical data
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Sensitivity and Specificity
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Shellfish / virology*