The information on the relation between the gonadal aspartate transcarbamylase (ACTase) activity and the sexual maturation in molluscs is very fragmentary and is still absent in Mya arenaria. The determination of ATCase activity, energy reserves levels and maturation stages were done in gonads of clams. Results showed that the seasonal cycle of storage and utilization of energy reserves in gonads of clams are linked to the bimodal reproduction well known in this bivalve. ATCase activity was high in clams at development and ripe stages, while this activity was low for individuals found in indifferent, spawning or spent stages. This difference can be explained by the fact that during gonad development, gonadal synthetic activity increased following the mitotic events associated to the reproductive cycle. The results presented in this paper have confirmed the link between ATCase activity and gametogenesis in M. arenaria. Further work should be realized in order to assess if ATCase activity could be considered as a potential biomarker to evaluate the disruption of sexual maturation in clams collected in sites such as the St. Lawrence estuary.