Efficient inoculation with CaMV 35 S promoter-driven DNA clones of the tobravirus PEBV

S A MacFarlane; D Gilmer; J W Davies

doi:10.1016/0042-6822(92)90488-b

Efficient inoculation with CaMV 35 S promoter-driven DNA clones of the tobravirus PEBV

Virology. 1992 Apr;187(2):829-31. doi: 10.1016/0042-6822(92)90488-b.

Authors

S A MacFarlane¹, D Gilmer, J W Davies

Affiliation

¹ Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, United Kingdom.

PMID: 1546470
DOI: 10.1016/0042-6822(92)90488-b

Abstract

Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Blotting, Northern
Cloning, Molecular
DNA / genetics
DNA Mutational Analysis
Gene Expression Regulation, Viral
Molecular Sequence Data
Oligodeoxyribonucleotides / chemistry
Plant Viruses / genetics*
Promoter Regions, Genetic
RNA, Viral / genetics
Transcription, Genetic

Substances

Oligodeoxyribonucleotides
RNA, Viral
DNA