Assessment of CFTR localisation in native airway epithelial cells obtained by nasal brushing

Ceinwen M Harris; Filipa Mendes; Anca Dragomir; Iolo J M Doull; I Carvalho-Oliveira; Zsuzsanna Bebok; John P Clancy; Valerie Eubanks; Eric J Sorscher; Godfried M Roomans; Margarida D Amaral; Margaret A McPherson; Deborah Penque; Robert L Dormer

doi:10.1016/j.jcf.2004.05.009

Assessment of CFTR localisation in native airway epithelial cells obtained by nasal brushing

J Cyst Fibros. 2004 Aug:3 Suppl 2:43-8. doi: 10.1016/j.jcf.2004.05.009.

Authors

Ceinwen M Harris¹, Filipa Mendes, Anca Dragomir, Iolo J M Doull, I Carvalho-Oliveira, Zsuzsanna Bebok, John P Clancy, Valerie Eubanks, Eric J Sorscher, Godfried M Roomans, Margarida D Amaral, Margaret A McPherson, Deborah Penque, Robert L Dormer

Affiliation

¹ Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff, Wales CF14 4XN, UK.

PMID: 15463924
DOI: 10.1016/j.jcf.2004.05.009

Abstract

Reliable methods for determining the localisation of mutant CFTR protein in native cells from CF individuals are necessary to allow the degree of mislocalisation of any genotype to be defined and to assess the effect of therapeutic agents on CFTR trafficking. Here, we present procedures for obtaining ciliated epithelial cells from CF patients by nasal brushing and a description of protocols for immunolocalisation of CFTR. The protocols are a consensus, following comparison of some aspects of methods currently used in the authors' laboratories.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't
Review

MeSH terms

Cystic Fibrosis / diagnosis*
Cystic Fibrosis / genetics
Cystic Fibrosis Transmembrane Conductance Regulator / genetics
Cystic Fibrosis Transmembrane Conductance Regulator / isolation & purification*
Epithelial Cells / chemistry
Histocytological Preparation Techniques / methods*
Humans
Respiratory Mucosa / chemistry
Respiratory Mucosa / pathology*
Specimen Handling / methods

Substances

CFTR protein, human
Cystic Fibrosis Transmembrane Conductance Regulator