Abstract
The Escherichia coli cell-free protein synthesis system can now be used for various proteins that need special requirements, such as disulfide bond formation between intra- and inter-molecules, hetero-dimerization, and specific chaperons. In addition, a novel protein library construction method termed "SIMPLEX" has been developed. Some applications with SIMPLEX are described. A highly efficient eukaryotic cell-free translation system using wheat germ extract has also been developed. An advanced framework for a rapid eukaryotic gene expression and analysis is shown.
MeSH terms
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5' Untranslated Regions / genetics
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5' Untranslated Regions / metabolism
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics
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Base Sequence
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Cell-Free System / metabolism
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DNA, Complementary / genetics
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Gene Expression / genetics
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Genomics / methods
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Molecular Sequence Data
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Peptide Library
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Plant Extracts / metabolism
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Polymerase Chain Reaction
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Potyviridae / genetics
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Protein Biosynthesis / genetics
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Protein Engineering / methods
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Proteomics / methods
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Sequence Homology
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Triticum / chemistry
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Triticum / genetics
Substances
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5' Untranslated Regions
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Bacterial Proteins
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DNA, Complementary
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Peptide Library
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Plant Extracts