[Transcriptome analysis of virus-infected cells]

Uirusu. 2004 Jun;54(1):23-31. doi: 10.2222/jsv.54.23.
[Article in Japanese]

Abstract

Human genome project revealed that human genes are derived from 30,000-40,000 species of genetic loci, which had been estimated as approximately 100,000. The project also promoted devising novel tools that enable us to analyze biological phenomena comprehensively. Microarray technology is a representative of the novel tools in genomics and engages us to explore genome-wide expression levels of genes simultaneously (transcriptome analysis). Here we show transcriptomes obtained from 10 species of cells infected with human cytomegalovirus, as a model virus, by a synthetic DNA microarray system that we have established recently. Our system provides simultaneous and parallel description on alteration of expression of viral and host genes that are represented within a single area on a slide glass. Moreover, we propose a project entitled 'comparative virology on cellular responses of infected hosts' that consists of multiple acquisition and integration of transcriptomes from a combination of several cells and viruses as a panel on the identical platform. The attempt should extract a novel concept in virology from investigation on differences and similarities among influence of a virus on a variety of different cells and conversely among responses of a species of cells against a variety of different viruses.

Publication types

  • English Abstract
  • Review

MeSH terms

  • Cytomegalovirus / genetics*
  • Cytomegalovirus Infections / genetics*
  • Cytomegalovirus Infections / immunology
  • Cytomegalovirus Infections / virology*
  • Gene Expression / genetics*
  • Gene Expression Profiling / methods*
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Transcription, Genetic / genetics*