Optical coherence tomography of malignancy in hamster cheek pouches

J Biomed Opt. 2004 Sep-Oct;9(5):978-81. doi: 10.1117/1.1783897.

Abstract

Optical coherence tomography (OCT)/optical Doppler tomography (ODT) provides real-time in vivo high-resolution (10-microm) imaging of tissues and real-time spatially resolved blood flow in microvasculature. Hamster cheek pouches with induced dysplasia and malignancies were imaged with OCT/ODT to assess the potential for application to airway malignancy. In 22 Golden Syrian hamsters, 0.5% 9,10-dimethyl-1,2-benzanthracene induces carcinogenesis over 10 weeks in right side cheek pouches; the left side three served as controls. The cheek pouches are imaged in vivo prior to sacrifice, and in vitro after excision, using a prototype 1310-nm broadband superluminescent diode based OCT/ODT device. Images are compared to standard histopathology. OCT imaging offers good resolution of the hamster cheek pouches to depths of 1 to 3 mm and paralleled histologic images. The feasibility of high-resolution functional imaging is demonstrated in this hamster cheek pouch tumor model. ODT accurately detects vascular change associated with carcinogenesis.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Animals
  • Cricetinae
  • Mesocricetus
  • Mouth Neoplasms / chemically induced
  • Mouth Neoplasms / pathology*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Single-Blind Method
  • Tomography, Optical Coherence / methods*

Substances

  • 9,10-Dimethyl-1,2-benzanthracene