Two-photon laser scanning fluorescence microscopy using photonic crystal fiber

J Biomed Opt. 2004 Sep-Oct;9(5):922-7. doi: 10.1117/1.1778734.

Abstract

We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiation of less than 35 fs duration reaching the sample.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Crystallization / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Fiber Optic Technology / instrumentation*
  • Fiber Optic Technology / methods
  • Guinea Pigs
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / methods
  • Muscle, Smooth, Vascular / ultrastructure*
  • Photons
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity