Abstract
We report the application of a simple yet powerful modular pulse compression system based on photonic crystal fibers that improves on incumbent two-photon laser scanning fluorescence microscopy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of IR radiation of less than 35 fs duration reaching the sample.
(c) 2004 Society of Photo-Optical Instrumentation Engineers.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Animals
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Crystallization / methods
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Equipment Design
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Equipment Failure Analysis
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Fiber Optic Technology / instrumentation*
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Fiber Optic Technology / methods
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Guinea Pigs
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Microscopy, Confocal / instrumentation*
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Microscopy, Confocal / methods
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Microscopy, Fluorescence, Multiphoton / instrumentation*
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Microscopy, Fluorescence, Multiphoton / methods
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Muscle, Smooth, Vascular / ultrastructure*
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Photons
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Rats
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Reproducibility of Results
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Sensitivity and Specificity