Isolation and partial characterization of molecular forms of ceruloplasmin from human bile

I A Verbina; L V Puchkova; V S Gaitskhoki; S A Neifakh

doi:10.1016/0014-5793(92)80032-c

Isolation and partial characterization of molecular forms of ceruloplasmin from human bile

FEBS Lett. 1992 Feb 24;298(2-3):105-8. doi: 10.1016/0014-5793(92)80032-c.

Authors

I A Verbina¹, L V Puchkova, V S Gaitskhoki, S A Neifakh

Affiliation

¹ Institute for Experimental Medicine, USSR Academy of Medical Sciences, Saint Petersburg.

PMID: 1544429
DOI: 10.1016/0014-5793(92)80032-c

Abstract

Highly purified ceruloplasmin (CP) was isolated from human bile using affinity chromatography. Biliary CP is represented by two molecular species. One of those is identical to oxidase CP from normal human serum while the other is analogous to oxidase-lacking CP specific for the serum of the carriers of Wilson's mutation with respect to immunological specificity, electrophoretical mobility and molecular mass of the large fragments from spontaneous proteolysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bile / chemistry*
Ceruloplasmin / chemistry
Ceruloplasmin / isolation & purification
Ceruloplasmin / metabolism*
Chromatography, Affinity
Copper / metabolism
Electrophoresis
Hepatolenticular Degeneration / blood
Humans
Immunoblotting

Substances

Copper
Ceruloplasmin