Abstract
A novel rapidly growing, non-pigmented mycobacterium was isolated from blood samples obtained from 17 patients with febrile syndrome. Bacterial growth occurred at 30 and 37 degrees C on Löwenstein-Jensen medium and also on MacConkey agar without crystal violet. Strains contained alpha- and alpha'-mycolates in their cell wall. Sequence analysis of the hsp65 and 16S rRNA genes identified the isolates as rapidly growing mycobacteria. Sequences of both genes were unique within the mycobacteria. DNA-DNA hybridization showed that the isolates had less than 15 % reassociation with 13 other recognized rapidly growing mycobacteria. The name Mycobacterium canariasense sp. nov. is proposed for this novel opportunistic pathogen, which is most closely related to Mycobacterium diernhoferi. The type strain is 502329T (= CIP 107998T = CCUG 47953T).
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / genetics
-
Bacterial Typing Techniques
-
Blood / microbiology
-
Cell Wall / chemistry
-
Chaperonin 60
-
Chaperonins / genetics
-
Culture Media
-
DNA Fingerprinting
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / isolation & purification
-
DNA, Ribosomal / chemistry
-
DNA, Ribosomal / isolation & purification
-
Genes, rRNA
-
Humans
-
Molecular Sequence Data
-
Mycobacterium / classification*
-
Mycobacterium / isolation & purification*
-
Mycobacterium / physiology
-
Mycobacterium Infections / microbiology
-
Mycolic Acids / analysis
-
Nucleic Acid Hybridization
-
Phylogeny
-
Polymorphism, Restriction Fragment Length
-
RNA, Bacterial / genetics
-
RNA, Ribosomal, 16S / genetics
-
Sequence Analysis, DNA
-
Spain
-
Temperature
Substances
-
Bacterial Proteins
-
Chaperonin 60
-
Culture Media
-
DNA, Bacterial
-
DNA, Ribosomal
-
Mycolic Acids
-
RNA, Bacterial
-
RNA, Ribosomal, 16S
-
heat-shock protein 65, Mycobacterium
-
Chaperonins
Associated data
-
GENBANK/AY255477
-
GENBANK/AY255478