Separation of polyethylene glycols and their amino-substituted derivatives by high-performance gel filtration chromatography at low ionic strength with refractive index detection

Min Liu; Cao Xie; Wen Xu; Weiyue Lu

doi:10.1016/j.chroma.2004.06.005

Separation of polyethylene glycols and their amino-substituted derivatives by high-performance gel filtration chromatography at low ionic strength with refractive index detection

J Chromatogr A. 2004 Aug 13;1046(1-2):121-6. doi: 10.1016/j.chroma.2004.06.005.

Authors

Min Liu¹, Cao Xie, Wen Xu, Weiyue Lu

Affiliation

¹ Department of Pharmaceutics, Fudan-Pharmco Targeting Drug Research Center, School of Pharmacy, University of Fudan, Shanghai 200032, China.

PMID: 15387179
DOI: 10.1016/j.chroma.2004.06.005

Abstract

The chromatographic analysis of polyethylene glycols (PEG) and their amino-substituted derivatives, with an average molecular mass of 2000 and 3350 is described. The PEG derivatives were perfectly separated by TSK-GEL G4000PW(XL) column, which was widely used in high-performance size-exclusion chromatography (SEC), at low ionic strength with refractive index detection. It was shown that ion-exchange interactions were mainly involved in the retention mechanism of these compounds on TSK-GEL G4000PW(XL) column, since retention volume decreased when salt concentration were added to the mobile phase and varied with the pH of the eluent. Additionally, size exclusion for PEG chains plays a role. Organic modifiers also had effect on chromatographic behaviors of PEG compounds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amines / chemistry*
Chromatography, Gel / methods*
Chromatography, High Pressure Liquid / methods*
Hydrogen-Ion Concentration
Osmolar Concentration
Polyethylene Glycols / chemistry
Polyethylene Glycols / isolation & purification*
Refractometry / methods*

Substances

Amines
Polyethylene Glycols