A new method for quantitative cellular imaging on 3-D scaffolds using fluorescence microscopy

IEEE Trans Nanobioscience. 2003 Jun;2(2):110-7. doi: 10.1109/tnb.2003.813938.

Abstract

This paper presents a new image processing technique for estimating cell numbers and contours in three-dimensional (3-D) microfabricated scaffolds. The method is based on a statistical approach, and utilizes the extreme value theory, which assumes that cells in the image field are rare, high-intensity events. Confocal microscopy images of fibroblasts on 3-D structures were processed using the method, and the resulting data on cell numbers was compared with countings obtained using a Burker chamber. The results were identical to within a few percent.

Publication types

  • Comparative Study
  • Evaluation Study
  • Validation Study

MeSH terms

  • 3T3 Cells
  • Algorithms*
  • Animals
  • Cell Count / methods*
  • Cell Culture Techniques / methods*
  • Cell Size
  • Image Interpretation, Computer-Assisted / methods*
  • Imaging, Three-Dimensional / methods*
  • Membranes, Artificial
  • Mice
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tissue Engineering / methods

Substances

  • Membranes, Artificial