Abstract
Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1 alpha) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1 alpha were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1 alpha sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA, Protozoan / chemistry
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DNA, Protozoan / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics*
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DNA, Ribosomal / isolation & purification
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Feces / parasitology
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Genotype
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Giardia / genetics*
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Giardia / isolation & purification
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Giardia / physiology
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Giardiasis / epidemiology
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Giardiasis / parasitology*
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Giardiasis / veterinary
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Host-Parasite Interactions
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Marsupialia / parasitology*
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Peptide Elongation Factor 1 / genetics*
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Phylogeny
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Sequence Analysis, DNA / veterinary
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Sequence Homology, Nucleic Acid
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Western Australia / epidemiology
Substances
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DNA, Protozoan
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DNA, Ribosomal
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Peptide Elongation Factor 1