The interaction between osteoclast-like cells and osteoblasts mediated by nanophase calcium phosphate-hybridized tendons

Biomaterials. 2005 Mar;26(9):1027-34. doi: 10.1016/j.biomaterials.2004.03.039.

Abstract

We developed a novel technique of hybridizing calcium phosphate (CaP) with bioorganic soft tissue using an alternating soaking process. By this technique, we hybridized CaP with a grafted tendon tissue to bond with a bone tunnel. Tendons were soaked in Ca and NaHPO(4) solutions alternately for 10 min. Needle-like CaP crystals 30-50 nm in length including low-crystalline apatite were deposited on and between collagen fibrils from the surface to 200 microm deep in the tendon. In light and transmission electron microscopic images, osteoclast-like cells and osteoblasts appeared on the implanted tendon and osteoid was observed on the tendon surface at 1 week postoperatively. At 2 weeks postoperatively, osteoclast-like cells resolved the tendon by forming Howship's lacuna-like spaces on the surfaces and osteoblasts formed osteoid in these spaces. Direct bonding between the implanted tendon and the newly formed bone was observed. At 3 weeks postoperatively, thick newly formed bone firmly bonded to tendon surface. From these results, we conclude that the tendons prepared by an accelerated CaP hybridization method efficiently enhance osteoclast-like cells and osteoblasts to bond the implanted tendons to newly formed bone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Phosphates / chemistry*
  • Male
  • Nanostructures / chemistry
  • Nanostructures / ultrastructure
  • Osseointegration / physiology
  • Osteoblasts / cytology*
  • Osteoblasts / physiology
  • Osteoclasts / cytology*
  • Osteoclasts / physiology
  • Phase Transition
  • Rabbits
  • Tendons / chemistry
  • Tendons / cytology*
  • Tendons / transplantation*
  • Tissue Engineering / methods*
  • Transplants*

Substances

  • Calcium Phosphates
  • calcium phosphate