Nucleation of calcium phosphate by surface-bound extracellular matrix

J Biomed Mater Res A. 2004 Oct 1;71(1):16-24. doi: 10.1002/jbm.a.30113.

Abstract

The native extracellular matrix (ECM) laid down on silicon and titanium surfaces by osteoblast-like SAOS-2 cells was exposed by selective removal of cells. This type of material surface ECM-Si, ECM-Ti was shown to promote the nucleation of calcium phosphate from a simulated body fluid (SBF). Microscopic and spectroscopic results revealed the effect was associated with a collagen fiber-free extracellular matrix.

MeSH terms

  • Body Fluids / chemistry
  • Calcium Phosphates / chemistry*
  • Calcium Phosphates / metabolism
  • Cell Line, Tumor
  • Coated Materials, Biocompatible / chemistry*
  • Coated Materials, Biocompatible / metabolism
  • Collagen / metabolism
  • Extracellular Matrix / chemistry*
  • Extracellular Matrix / metabolism
  • Humans
  • Materials Testing
  • Microscopy, Atomic Force
  • Microscopy, Electron
  • Silicon / chemistry
  • Silicon / metabolism
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties
  • Titanium / chemistry
  • Titanium / metabolism
  • X-Ray Diffraction

Substances

  • Calcium Phosphates
  • Coated Materials, Biocompatible
  • Collagen
  • calcium phosphate
  • Titanium
  • Silicon