Comparison of multiplexed techniques for detection of bacterial and viral proteins

Rupa S Rao; Steven R Visuri; Mary T McBride; Joanna S Albala; Dennis L Matthews; Matthew A Coleman

doi:10.1021/pr034130t

Comparison of multiplexed techniques for detection of bacterial and viral proteins

J Proteome Res. 2004 Jul-Aug;3(4):736-42. doi: 10.1021/pr034130t.

Authors

Rupa S Rao¹, Steven R Visuri, Mary T McBride, Joanna S Albala, Dennis L Matthews, Matthew A Coleman

Affiliation

¹ Lawrence Livermore National Laboratory, Livermore, California 94550, USA.

PMID: 15359726
DOI: 10.1021/pr034130t

Abstract

Immobilized antibody microarrays were compared to the Luminex flow cytometry system that utilizes suspensions of polystyrene microbeads covalently coupled with capture antibodies. The two immunoassays were performed for comparison of reproducibility, limits of detection and dynamic range. The Luminex system showed lower limits of detection and increased dynamic range among samples whereas the protein microarrays could be more amenable to miniaturization. Both technologies were capable of sensitive multiplexed detection.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Antibodies, Bacterial / chemistry
Antibodies, Bacterial / immunology
Antibodies, Viral / chemistry
Antibodies, Viral / immunology
Bacillus / metabolism
Bacterial Proteins / analysis*
Bacterial Proteins / immunology
Flow Cytometry
Levivirus / metabolism
Microspheres
Protein Array Analysis / methods*
Viral Proteins / analysis*
Viral Proteins / immunology

Substances

Antibodies, Bacterial
Antibodies, Viral
Bacterial Proteins
Viral Proteins