Synthesis and cellular uptake of a fluorescently labeled cyclic PNA-based compound

Sergio Caldarelli; Geoffrey Depecker; Nadia Patino; Audrey Di Giorgio; Thibault Barouillet; Alain Doglio; Roger Condom

doi:10.1016/j.bmcl.2004.06.055

Synthesis and cellular uptake of a fluorescently labeled cyclic PNA-based compound

Bioorg Med Chem Lett. 2004 Sep 6;14(17):4435-8. doi: 10.1016/j.bmcl.2004.06.055.

Authors

Sergio Caldarelli¹, Geoffrey Depecker, Nadia Patino, Audrey Di Giorgio, Thibault Barouillet, Alain Doglio, Roger Condom

Affiliation

¹ Laboratoire de Chimie Bioorganique UMR-CNRS 6001, Université de Nice-Sophia Antipolis, Parc Valrose, 06108 Cédex 2, France.

PMID: 15357967
DOI: 10.1016/j.bmcl.2004.06.055

Abstract

A cyclic hexameric PNA-based compound labeled with fluorescein has been prepared following the liquid phase FPB strategy. Its cellular uptake, without and with electroporation, has been investigated by fluorescence microscopy.

MeSH terms

Cell Membrane / chemistry
Cell Membrane / metabolism*
Fluorescein / analysis
Fluorescein / metabolism*
HeLa Cells
Humans
Jurkat Cells
Nucleic Acid Conformation*
Nylons / analysis
Nylons / chemical synthesis*
Nylons / metabolism
RNA / analysis
RNA / chemical synthesis*
RNA / metabolism

Substances

Nylons
RNA
Fluorescein