Substantial progress has been made in the lat two decades to evaluate the impact of physical and chemical genotoxins in aquatic organisms. This overview (a) summarises the major high lights in this stimulating area of research, (b) compares the developments in this field with the developments in mammalian genotoxicological studies, where appropriate, (c) introduces 18 different articles presented in this special issue of Mutation Research in the backdrop of main advances and , (d) hypothesises on future directions of research in this exciting field.