[Construction of replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM genes of human immunodeficiency virus in mice]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004 Jun;18(2):150-3.
[Article in Chinese]

Abstract

Background: Construction of replication-deficient recombinant adenovirus expressing gag-pol and env genes of human immunodeficiency virus (HIV) in mice.

Methods: gag-polDelta and gp140TM genes were cloned into shuttle vector pAdTrack-CMV respectively, and then the plasmids containing gag-polDelta or gp140TM gene were cotransformed with the backbone of adenovirus into E.coli BJ5183. Transfections of the recombinants were performed to obtain recombinant adenoviruses. Its immunogenicity was evaluated by testing antibody levels of mice primed with DNA vaccines and boosted with recombinant adenoviruses.

Results: The replication-deficient recombinant adenovirus could express Gp140TM, Gag P55 and P24 proteins correctly. The mice primed with DNA vaccines and boosted with recombinant adenoviruses elicited high titer of HIV-1-specific antibody compared with that inoculated with DNA vaccines only.

Conclusion: Replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM can elicit high titer HIV-1-specific antibodies.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / immunology*
  • Adenoviridae / genetics*
  • Animals
  • Female
  • Fusion Proteins, gag-pol / biosynthesis
  • Fusion Proteins, gag-pol / genetics*
  • Gene Products, env / biosynthesis
  • Gene Products, env / genetics*
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Recombination, Genetic
  • Transfection
  • Vaccines, DNA / immunology*
  • env Gene Products, Human Immunodeficiency Virus

Substances

  • AIDS Vaccines
  • Fusion Proteins, gag-pol
  • Gene Products, env
  • Vaccines, DNA
  • env Gene Products, Human Immunodeficiency Virus
  • gp140 envelope protein, Human immunodeficiency virus 1