Abstract
For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5'-carbonate protected 2'-deoxynucleoside-3'-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate protecting group and oxidizes the internucleotide linkage. Following synthesis via this two-step cycle, oligomers are isolated by standard procedures.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amides / chemistry
-
Chromatography, High Pressure Liquid
-
DNA / chemical synthesis*
-
Electrophoresis, Polyacrylamide Gel
-
Indicators and Reagents / chemistry
-
Magnetic Resonance Spectroscopy
-
Oxidation-Reduction
-
Phosphoric Acids / chemistry
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
-
Amides
-
Indicators and Reagents
-
Phosphoric Acids
-
DNA
-
phosphoramidic acid