Local genetic patterns within a vancomycin-resistant Enterococcus faecalis clone isolated in three hospitals in Portugal

Antimicrob Agents Chemother. 2004 Sep;48(9):3613-7. doi: 10.1128/AAC.48.9.3613-3617.2004.

Abstract

Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Chromosomes / chemistry
  • Chromosomes / genetics
  • Cross Infection / epidemiology
  • Cross Infection / microbiology*
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Enterococcus faecalis / drug effects
  • Enterococcus faecalis / genetics*
  • Gram-Positive Bacterial Infections / epidemiology
  • Gram-Positive Bacterial Infections / microbiology*
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Portugal / epidemiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vancomycin / pharmacology
  • Vancomycin Resistance / genetics*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Vancomycin