Abstract
MALDI-TOF MS and N-terminal amino acid sequencing allowed us to identify several fragments of the C-terminal peptide of Influenza A hemagglutinin (HA) containing transmembrane domains (TMD). These fragments were detected in the organic phase of chloroform-methanol extracts from bromelain-treated virus particles. Heterogeneous fatty acylation of the C-terminus was revealed. Tritium bombardment technique might open an opportunity for 3D structural investigation of the HA TMD in situ.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Bromelains
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Chick Embryo
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Chloroform
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Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
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Hemagglutinin Glycoproteins, Influenza Virus / isolation & purification
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism*
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Influenza A virus / chemistry*
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Methanol
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Molecular Sequence Data
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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Chloroform
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Bromelains
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Methanol