Abstract
A versatile microplate bioassay for quick and sensitive determination of antibacterial activity was developed for use in screening medicinal plants and identification of their active principles. This assay can be used to determine minimum inhibitory concentrations for small quantities of organic or water-soluble plant extracts. Bioassay-guided fractionation of the stem and leaves of Peperomia galioides using this method found fractions containing grifolin and grifolic acid, which inhibited growth of Staphylococcus aureus and Staphylococcus epidermidis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Microbial Sensitivity Tests / methods*
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Microbial Sensitivity Tests / statistics & numerical data
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Peperomia*
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Peru
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Leaves
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Plant Stems
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Plants, Medicinal*
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / growth & development
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Staphylococcus epidermidis / drug effects
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Staphylococcus epidermidis / growth & development
Substances
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Anti-Bacterial Agents
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Plant Extracts