Abstract
The Bacillus subtilis lmrAB operon is involved in multidrug resistance. LmrA is a repressor of its own operon, while LmrB acts as a multidrug efflux transporter. LmrA was produced in Escherichia coli cells and was shown to bind to the lmr promoter region, in which an LmrA-binding site was identified. Genome-wide screening involving DNA microarray analysis allowed us to conclude that LmrA also repressed yxaGH, which was not likely to contribute to the multidrug resistance. LmrA bound to a putative yxaGH promoter region, in which two tandem LmrA-binding sites were identified. The LmrA regulon was thus determined to comprise lmrAB and yxaGH. All three LmrA-binding sites contained an 18-bp consensus sequence, TAGACCRKTCWMTATAWT, which could play an important role in LmrA binding.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / genetics*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Bacterial Proteins / physiology
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Base Sequence
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Binding Sites
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Cloning, Molecular
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Consensus Sequence
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression Profiling
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Gene Expression Regulation, Bacterial*
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Molecular Sequence Data
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Multidrug Resistance-Associated Proteins / genetics
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Multidrug Resistance-Associated Proteins / physiology*
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Oligonucleotide Array Sequence Analysis
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Operon
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Promoter Regions, Genetic
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Protein Binding
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Regulon
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Repressor Proteins / physiology*
Substances
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Bacterial Proteins
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Multidrug Resistance-Associated Proteins
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Recombinant Proteins
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Repressor Proteins