Abstract
Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, a significant and equivalent anti-BRSV G IgG response was elicited to the full-length and truncated BRSV G proteins. These constructs may be used to study host pathological and immunological responses.
MeSH terms
-
Animals
-
Antibodies, Viral / analysis
-
Antibodies, Viral / biosynthesis*
-
COS Cells
-
Cattle
-
Cells, Cultured
-
Chlorocebus aethiops
-
Conserved Sequence
-
DNA / biosynthesis
-
DNA / genetics
-
DNA, Viral / analysis
-
DNA, Viral / biosynthesis
-
GTP-Binding Proteins / biosynthesis*
-
GTP-Binding Proteins / genetics
-
GTP-Binding Proteins / immunology*
-
Genetic Vectors
-
Immunoglobulin G / analysis
-
Immunoglobulin G / biosynthesis
-
Mice
-
Mice, Inbred BALB C
-
Neutralization Tests
-
Plasmids / genetics
-
Plasmids / immunology
-
Respiratory Syncytial Virus, Bovine / immunology*
-
Respiratory Syncytial Virus, Bovine / metabolism*
-
Transfection
-
Vaccines, Subunit / biosynthesis
-
Vaccines, Subunit / immunology
-
Vaccines, Synthetic / biosynthesis
-
Vaccines, Synthetic / immunology
-
Viral Vaccines / biosynthesis*
-
Viral Vaccines / immunology*
Substances
-
Antibodies, Viral
-
DNA, Viral
-
Immunoglobulin G
-
Vaccines, Subunit
-
Vaccines, Synthetic
-
Viral Vaccines
-
DNA
-
GTP-Binding Proteins