Single nucleotide polymorphism typing on DNA array with hydrophobic surface fabricated by plasma-polymerization technique

Biosens Bioelectron. 2004 Sep 15;20(2):184-9. doi: 10.1016/j.bios.2004.02.022.

Abstract

A DNA array has been fabricated on glass substrates, which enables high-throughput analysis of single-base mismatches. In this work, microfabrication-compatible plasma-polymerization (PP) method was used for immobilizing probe DNAs to study the hybridization behavior by changing surface properties. The immobilization matrix consisting of 35 A of PP layer, applied additionally on the streptavidin absorbed hexamethyldisiloxane (HMDS)-PP layer, was constructed on the substrates to anchor biotinylated DNA probes onto the surface. The hydrophobic immobilization matrix was considered to enhance hybridization accuracy and efficiency, compared with its hydrophilic acetonitrile-PP layers. The oligonucleotide arrays fabricated on HMDS-PP surface were shown to be effective in detection of single nucleotide polymorphisms (SNPs) of ApoE gene.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apolipoproteins E / analysis
  • Apolipoproteins E / genetics
  • Coated Materials, Biocompatible / chemistry
  • DNA Mutational Analysis / instrumentation
  • DNA Mutational Analysis / methods*
  • DNA Probes / chemistry*
  • Equipment Design
  • Equipment Failure Analysis
  • Gene Expression Profiling / instrumentation
  • Gene Expression Profiling / methods*
  • Hot Temperature
  • Hydrophobic and Hydrophilic Interactions
  • Manufactured Materials
  • Oligonucleotide Array Sequence Analysis / instrumentation
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction / methods*
  • Polymers / chemistry
  • Polymorphism, Single Nucleotide / genetics*

Substances

  • Apolipoproteins E
  • Coated Materials, Biocompatible
  • DNA Probes
  • Polymers