The objective of this study was to determine harvest yield and essential oil composition of six hop cultivars ("Fredos derlingieji", "Alta", "Granit", "Marynka", "Dubskij zeleniak" and "Aromat polessja") cultivated in Lithuania. It was found that the highest crop can be obtained from early and middle harvest varieties, which mature during the first decade of September and the third decade of August. Late harvest cultivars are not suitable for cultivation, however they can be used for the development of new cultivars. Essential oils were isolated by hydrodistillation and analyzed by gas chromatography and mass spectrometry. The highest amount of essential oil was determined in Marynka cultivar (2.10 ml/100 g), the lowest one in Dubskij zeleniak 18 (0.46 ml/100 g). Totally, 62 compounds were identified in all cultivars. Myrcene, beta-caryophyllene, alpha-humulene and beta-farnesene (E) were major constituents in the essential oils. The differences in the content of other essential oil constituents were determined. Some cultivars were rich in esters and ketones.