Crystallization and preliminary X-ray diffraction studies of the Pseudomonas marginata esterase EstB

U G Wagner; B Sölkner; E I Petersen; A Schlacher; H Schwab; C Kratky

doi:10.1107/S0907444997004988

Crystallization and preliminary X-ray diffraction studies of the Pseudomonas marginata esterase EstB

Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):596-8. doi: 10.1107/S0907444997004988.

Authors

U G Wagner¹, B Sölkner, E I Petersen, A Schlacher, H Schwab, C Kratky

Affiliation

¹ Abteilung für Strukturbiologie, Institut für Physikalische Chemie, Karl-Franzens-Universität Graz, Austria. ulrike.wagner@kfunigraz.ac.at

PMID: 15299891
DOI: 10.1107/S0907444997004988

Abstract

Crystals of the esterase EstB were obtained at 277 K with the hanging-drop technique from polyethylene glycol 4000 solutions containing 2-propanol at pH 7.5. The crystals belong to the trigonal space group P3(1)21 (or P3(2)21) with cell dimensions a = b = 82.9 and c = 193.4 A (at 100 K). The crystals diffract beyond a resolution of 2.0 A.