Preliminary X-ray diffraction study of a new crystal form of C-1027-AG, the apoprotein of the macromolecular antitumor antibiotic C-1027 from Streptomyces globisporus

F Motojima; K Inaka; Y Minami; T Otani; K Miki

doi:10.1107/S0907444995004197

Preliminary X-ray diffraction study of a new crystal form of C-1027-AG, the apoprotein of the macromolecular antitumor antibiotic C-1027 from Streptomyces globisporus

Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):1084-5. doi: 10.1107/S0907444995004197.

Authors

F Motojima¹, K Inaka, Y Minami, T Otani, K Miki

Affiliation

¹ Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.

PMID: 15299781
DOI: 10.1107/S0907444995004197

Abstract

A new crystal form of C-1027-AG, the apoprotein of the macromolecular antitumor antibiotic C-1027 isolated from Streptomyces globisporus was obtained by the vapor-diffusion procedure using lithium sulfate as a precipitant. In the present crystallization, it is noteworthy that large-sized single crystals successfully grew from very small droplets (less than 1.01 micro l). The present crystals belong to the trigonal system, space group P3(1)21 or P3(2)21 with cell dimensions of a = b = 62.6 and c = 54.2 A. Assuming that the asymmetric unit contains one molecule, the V(m) value is calculated as 2.9 A(3) Da(-1). A total of 3654 independent reflections from two native crystals was obtained up to 2.5 A resolution with synchrotron radiation, the merging R factor being 0.097.