Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation

J Clin Microbiol. 2004 Aug;42(8):3827-30. doi: 10.1128/JCM.42.8.3827-3830.2004.

Abstract

A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomyces / genetics
  • Actinomyces / isolation & purification
  • Actinomyces / physiology
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Bacterial Physiological Phenomena
  • Base Sequence
  • Biofilms*
  • Computer Systems
  • DNA Primers
  • Humans
  • Mouth / microbiology*
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Polymerase Chain Reaction / methods
  • Streptococcus / genetics
  • Streptococcus / isolation & purification
  • Streptococcus / physiology

Substances

  • DNA Primers
  • Oligonucleotide Probes