A description of the pathophysiology of aspergillosis is followed by a review of investigational considerations of animal models. Because a large body of invasive Aspergillus infection occurs as opportunistic infection, there is a large spectrum of the histopathological feature of lesions demonstrated at the site of infection. Histopathology of the lesions can be understood as a phenotypical representation of interaction between lowered defense mechanisms in the host and the virulence of invading fungi. Detailed observations with a consideration of previous pathological knowledge of infection and inflammation provide much important information useful in predicting the pathophysiology of the patient. Moreover, experimental studies can also provide much insight to elucidate pathogenesis of the infection that emerges from the clinical and pathological investigations. The importance of pathophysiology should be emphasized to understand the implications of radiographic images, clinical symptoms, and laboratory dates. By reviewing these, especially computed tomography (CT) images, we can see that they accurately mirror the histological features of the lesion that can be recognized as a phenotypical representation of pathophysiology of Aspergillus infection. This is also confirmed by the reports emphasizing the importance of CT scans to identify hallmark clinical signs and symptoms of the disease.