Imaging glucose-regulated insulin secretion and gene expression in single islet beta-cells: control by AMP-activated protein kinase

Cell Biochem Biophys. 2004;40(3 Suppl):179-90. doi: 10.1385/cbb:40:3:179.

Abstract

The mechanisms by which changes in glucose concentration regulate gene expression and insulin secretion in pancreatic islet beta-cells are only partly understood. Here we describe the development of new technologies for examining these processes at the level of single living beta-cells. We also present recent findings, made using these and other techniques, which implicate a role for adenosine 5'-monophosphate-activated protein kinase in glucose signaling in these cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • AMP-Activated Protein Kinases / metabolism*
  • Animals
  • Gene Expression Profiling*
  • Gene Expression Regulation
  • Glucose / metabolism*
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Insulin / metabolism*
  • Insulin Secretion
  • Insulin-Secreting Cells / cytology*
  • Insulin-Secreting Cells / metabolism
  • Mice
  • Microscopy, Fluorescence / methods
  • Models, Biological
  • Phosphatidylinositol 3-Kinases / metabolism
  • Signal Transduction
  • Time Factors

Substances

  • Insulin
  • Green Fluorescent Proteins
  • Phosphatidylinositol 3-Kinases
  • AMP-Activated Protein Kinases
  • Glucose