Antiinflammatory activity of hyperin from Acanthopanax chiisanensis roots

Arch Pharm Res. 2004 Jun;27(6):628-32. doi: 10.1007/BF02980162.

Abstract

The chloroform and the ethyl acetate fractions from the roots of Acanthopanax chiisanensis exhibited a significant inhibition of prostaglandin E2 (PGE2) production in rat peritoneal macrophages stimulated by the protein kinase C activator, 12-O-tetradecanoylphorbol 13-acetate (TPA). Hyperin was isolated as an active principle from the ethyl acetate fraction. It suppressed not only PGE2 production but also nitric oxide (NO) production in vitro in a concentration dependent manner, their IC50, being 24.3 and 32.9 microM, respectively. Hyperin also caused a significant inhibition of increase in acetic acid-induced vascular permeability in mice in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / chemistry
  • Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Blotting, Western
  • Capillary Permeability / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Eleutherococcus / chemistry*
  • Injections, Intraperitoneal
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / metabolism
  • Male
  • Mice
  • Nitric Oxide Synthase / metabolism
  • Nitrites / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plant Roots / chemistry
  • Quercetin / analogs & derivatives*
  • Quercetin / chemistry
  • Quercetin / isolation & purification
  • Quercetin / pharmacology*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Nitrites
  • Plant Extracts
  • hyperoside
  • Quercetin
  • Nitric Oxide Synthase