Abstract
The chloroform and the ethyl acetate fractions from the roots of Acanthopanax chiisanensis exhibited a significant inhibition of prostaglandin E2 (PGE2) production in rat peritoneal macrophages stimulated by the protein kinase C activator, 12-O-tetradecanoylphorbol 13-acetate (TPA). Hyperin was isolated as an active principle from the ethyl acetate fraction. It suppressed not only PGE2 production but also nitric oxide (NO) production in vitro in a concentration dependent manner, their IC50, being 24.3 and 32.9 microM, respectively. Hyperin also caused a significant inhibition of increase in acetic acid-induced vascular permeability in mice in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Blotting, Western
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Capillary Permeability / drug effects
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Cell Survival / drug effects
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Cells, Cultured
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Eleutherococcus / chemistry*
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Injections, Intraperitoneal
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Macrophages, Peritoneal / cytology
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Macrophages, Peritoneal / drug effects*
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Macrophages, Peritoneal / metabolism
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Male
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Mice
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Nitric Oxide Synthase / metabolism
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Nitrites / metabolism
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Roots / chemistry
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Quercetin / analogs & derivatives*
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Quercetin / chemistry
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Quercetin / isolation & purification
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Quercetin / pharmacology*
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Rats
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Rats, Sprague-Dawley
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Nitrites
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Plant Extracts
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hyperoside
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Quercetin
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Nitric Oxide Synthase