Marinibacillus campisalis sp. nov., a moderate halophile isolated from a marine solar saltern in Korea, with emended description of the genus Marinibacillus

Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1317-1321. doi: 10.1099/ijs.0.02779-0.

Abstract

A Gram-positive, motile, round to ellipsoidal, endospore-forming, rod-shaped bacterial strain, SF-57T, was isolated from a marine solar saltern in Korea. This organism grew between 4 and 39 degrees C, with optimum growth at 30 degrees C. Strain SF-57T grew in the presence of 0.5-15.0% NaCl, with optimum growth at 2-3% NaCl. The peptidoglycan type of strain SF-57T was A1alpha linked directly through l-Lys. In strain SF-57TT, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and anteiso-C(15 : 0) was the major fatty acid. The DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SF-57T formed a coherent cluster with Marinibacillus marinus, with a bootstrap resampling value of 100%. The level of 16S rRNA gene sequence similarity between strain SF-57T and M. marinus DSM 1297T was 98.9%. The mean DNA-DNA relatedness level between strain SF-57T and the type strain of M. marinus was 20.6%. Based on phenotypic properties, phylogenetic analyses and genomic data, strain SF-57T merits placement in the genus Marinibacillus as a representative of a novel species, for which the name Marinibacillus campisalis sp. nov. is proposed. The type strain is SF-57T (=KCCM 41644T=JCM 11810T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / classification*
  • Bacillaceae / cytology
  • Bacillaceae / isolation & purification*
  • Bacillaceae / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / chemistry
  • DNA, Ribosomal / chemistry
  • Fatty Acids / analysis
  • Genes, rRNA
  • Gentian Violet
  • Geologic Sediments / microbiology*
  • Growth Inhibitors / pharmacology
  • Korea
  • Molecular Sequence Data
  • Movement
  • Peptidoglycan / chemistry
  • Phenazines
  • Phylogeny
  • Quinones / analysis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology
  • Sodium Chloride / pharmacology
  • Spores, Bacterial / cytology
  • Temperature
  • Water Microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Gram's stain
  • Growth Inhibitors
  • Peptidoglycan
  • Phenazines
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Gentian Violet

Associated data

  • GENBANK/AY190535