Anoxybacillus voinovskiensis sp. nov., a moderately thermophilic bacterium from a hot spring in Kamchatka

Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1239-1242. doi: 10.1099/ijs.0.02889-0.

Abstract

A novel moderately thermophilic bacterium, strain TH13T, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30-64 degrees C (optimum 54 degrees C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for H2S production and growth in more than 3% NaCl (w/v). The isolate grew at pH 7-8, but not at pH values higher than 9. The DNA G+C content was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13T was a member of the genus Anoxybacillus. DNA-DNA hybridization revealed a low relatedness (less than 30.2%) between the isolate and its close phylogenetic neighbours Anoxybacillus pushchinoensis and Anoxybacillus flavithermus. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name Anoxybacillus voinovskiensis sp. nov. is proposed. The type strain of this species is TH13T (=NCIMB 13956T=JCM 12111T).

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Bacillaceae / classification*
  • Bacillaceae / cytology
  • Bacillaceae / isolation & purification*
  • Bacillaceae / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Ribosomal / chemistry
  • Genes, rRNA
  • Gentian Violet
  • Growth Inhibitors / pharmacology
  • Hot Springs / microbiology*
  • Hydrogen Sulfide / metabolism
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Movement
  • Nitrates / metabolism
  • Nucleic Acid Hybridization
  • Oxidation-Reduction
  • Oxidoreductases / metabolism
  • Phenazines
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Russia
  • Sequence Analysis, DNA
  • Sequence Homology
  • Sodium Chloride / pharmacology
  • Temperature
  • Water Microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Gram's stain
  • Growth Inhibitors
  • Nitrates
  • Phenazines
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Sodium Chloride
  • Oxidoreductases
  • Catalase
  • Gentian Violet
  • Hydrogen Sulfide

Associated data

  • GENBANK/AB110008