Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa

Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1467-9. doi: 10.1107/S0907444904013496. Epub 2004 Jul 21.

Abstract

FpvA, the pyoverdine outer-membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine-loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was collected at 3.6 A resolution and a three-wavelength MAD data set was collected at 3.6 A resolution using crystals of selenomethionine-substituted protein. The crystals grew under similar conditions and both belong to space group C2, but have different unit-cell parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry*
  • Bacterial Outer Membrane Proteins / metabolism
  • Crystallization
  • Crystallography, X-Ray
  • Oligopeptides / metabolism*
  • Pseudomonas aeruginosa / chemistry*
  • Pseudomonas aeruginosa / cytology

Substances

  • Bacterial Outer Membrane Proteins
  • FpvA protein, Pseudomonas aeruginosa
  • Oligopeptides
  • pyoverdin