[Skin-like structure generated from implantation of hair follicle bulb cells into collagen/chitosan porous scaffolds in vitro]

Xian-jie Wu; Zhong-fa Lu; Min Zheng; Yue-lan Chao; Sui-qing Cai; Jian-guang Zhou; Lie Ma; Chang-you Gao

doi:10.3785/j.issn.1008-9292.2004.04.002

[Skin-like structure generated from implantation of hair follicle bulb cells into collagen/chitosan porous scaffolds in vitro]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2004 Jul;33(4):281-6. doi: 10.3785/j.issn.1008-9292.2004.04.002.

[Article in Chinese]

Authors

Xian-jie Wu¹, Zhong-fa Lu, Min Zheng, Yue-lan Chao, Sui-qing Cai, Jian-guang Zhou, Lie Ma, Chang-you Gao

Affiliation

¹ Department of Dermatology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.

PMID: 15269975
DOI: 10.3785/j.issn.1008-9292.2004.04.002

Abstract

Objective: To observe the skin regeneration after hair follicle bulb cells were implanted into collagen/chitosan porous scaffolds in vitro.

Methods: The cultured dorsal hair follicle bulb cells of 4d-old C57BL/6J mice were implanted into collagen/chitosan porous scaffolds in vitro. The skin regeneration was observed.

Result: The skin-like structure was formed on the collagen/chitosan porous scaffolds where were cultured the hair follicle bulb cells before 4th passages.

Conclusion: The skin-like structure is generated in vitro when early passages of cultured hair bulb cells are implanted into collagen/chitosan porous scaffolds.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chitin / analogs & derivatives*
Chitosan
Collagen
Hair Follicle / cytology*
Mice
Mice, Inbred C57BL
Regeneration
Skin / cytology*
Tissue Engineering*

Substances

Chitin
Collagen
Chitosan