Abstract
Insertion sequences (IS) have been characterized in Microcystis aeruginosa gas vesicle-deficient mutants. ISMae4, a homolog of the cyanobacterial IS702, belongs to the IS5 family, subgroup ISL2. ISMae2 and ISMae3 display typical IS features and express a transposase of the IS4 and IS1 family, respectively. ISMae1 exhibits a more complex genetic structure and harbours a degenerated transposase gene distantly related to IS1 elements. Hybridizations with IS-specific DNA probes suggest that transposition of ISMae2 and ISMae3 occurred by a replicative-type mechanism. To our knowledge this is the first report showing that IS1 elements can be mobile in cyanobacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Conserved Sequence
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DNA Replication
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DNA Transposable Elements*
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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Gene Order
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Genes, Bacterial*
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Microcystis / genetics*
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Microcystis / metabolism
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Microcystis / ultrastructure
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Molecular Sequence Data
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Multigene Family
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Nucleic Acid Hybridization
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Proteins / genetics*
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Recombination, Genetic
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Sequence Alignment
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Sequence Analysis, DNA
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Transposases / chemistry
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Transposases / genetics
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Transposases / metabolism
Substances
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DNA Transposable Elements
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DNA, Bacterial
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Proteins
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gas vesicle protein
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Transposases
Associated data
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GENBANK/AJ577137
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GENBANK/AJ577138
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GENBANK/AJ577139
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GENBANK/AJ577140
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GENBANK/AJ697886