Screening of filamentous fungi for the decolorization of a commercial reactive dye

Abstract

The aim of this work is to verify the ability of 19 isolates of 13 different fungal species to decolorize the reactive dye blue-BF-R. The isolates of Pleurotus pulmonarius, P. ostreatus, P. ëous, P. citrinopileatus, Lentinus edodes, Phanerochaete chrysosporium, Schizophyllum commune, Agaricus blazei, Ganoderma sp. and four isolates obtained from textile effluent were evaluated in minimum liquid medium. In addition, seven of them were also evaluated on solid medium, and both media were both added 0.5 g dye/l. All isolates evaluated on solid medium decolorized the dye. The isolates Phanerochaete chrysosporium CCB478 and Lentinus edodes CCB047 were the ones that presented the fastest and slowest growth, respectively. Despite the isolate of the textile effluent had grown on solid medium, it did not decolorize the dye. All the isolates of the genus Pleurotus, except the isolate Pleurotus ëous CCB440, decolorized the dye in liquid medium. They presented decolorization percentage ranging from 39% to 51%. The absorbance ratio (Abs590/Abs455) of the culture medium inoculated with these isolates decreased throughout the experiment indicating the fungal dye degradation. The others presented decolorization percent below 8%. The isolates of Pleurotus, except the isolate Pleurotus ëous CCB440, were able to decolorize and to degrade the commercial reactive dye blue-BF-R. The results indicate their potential to be used in the treatment of effluents containing this dye.