Multienzyme system for dihydroxyacetone phosphate-dependent aldolase catalyzed C-C bond formation from dihydroxyacetone

Israel Sánchez-Moreno; Juan Francisco García-García; Agatha Bastida; Eduardo García-Junceda

doi:10.1039/b405220j

Multienzyme system for dihydroxyacetone phosphate-dependent aldolase catalyzed C-C bond formation from dihydroxyacetone

Chem Commun (Camb). 2004 Jul 21:(14):1634-5. doi: 10.1039/b405220j. Epub 2004 Jun 8.

Authors

Israel Sánchez-Moreno¹, Juan Francisco García-García, Agatha Bastida, Eduardo García-Junceda

Affiliation

¹ Departamento de Química Orgánica Biológica, Instituto de Química Orgánica General, CSIC, Madrid 28006, Spain.

PMID: 15263954
DOI: 10.1039/b405220j

Abstract

A multienzyme system composed by recombinant dihydroxyacetone kinase from Citrobacter freundii, fuculose-1-phosphate aldolase and acetate kinase, allows a practical one-pot C-C bond formation catalysed by dihydroxyacetone phosphate-dependent aldolases from dihydroxyacetone and an aldehyde.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Dihydroxyacetone / chemistry*
Dihydroxyacetone Phosphate / chemistry*
Escherichia coli / enzymology
Fructose-Bisphosphate Aldolase / chemistry*
Fructose-Bisphosphate Aldolase / metabolism
Kinetics
Molecular Structure
Multienzyme Complexes / metabolism*
Phosphotransferases (Alcohol Group Acceptor) / genetics

Substances

Multienzyme Complexes
Dihydroxyacetone Phosphate
Phosphotransferases (Alcohol Group Acceptor)
glycerone kinase
Fructose-Bisphosphate Aldolase
Dihydroxyacetone