Cadmium (Cd) is a toxic heavy metal which causes oxidative damage in organisms. In this study, we investigated the activities of the antioxidant enzymes erythrocyte catalase and copper/zinc superoxide dismutase (Cu/Zn-SOD) in 22 male inhabitants of the Cd-polluted Jinzu River basin in Toyama Prefecture, Japan (Cd group). The reference group consisted of 21 male inhabitants from an area that was not polluted with Cd (reference group). Urinary Cd levels and two renal tubular dysfunction markers in urine, alpha1-microglobulin (alpha1-m) level and N-acetyl-beta-D-glucosaminidase (NAG) activity, were significantly elevated in the Cd group. Catalase and Cu/Zn-SOD activities were significantly reduced in the Cd group as compared to the reference group. Significant negative correlation was observed between the activities of these two antioxidant enzymes and urinary Cd levels. We also observed significant negative correlations between activities of these two antioxidant enzymes and the renal tubular dysfunction markers. Our results indicate that erythrocyte catalase and Cu/Zn-SOD activities are reduced as a result of long-term Cd exposure. This may be linked to renal tubular dysfunction in the inhabitants of the Cd-polluted area.