Abstract
A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques. The purified peptide was applied to the MALDI-TOP MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121, was obtained, with the molecular mass of 510.8 Da and the sequence being "ACSAG".
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Chromatography, Gel
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Escherichia coli / drug effects
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Molecular Weight
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Oligochaeta / chemistry*
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Peptides / chemistry
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Peptides / isolation & purification*
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Peptides / pharmacology
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Pseudomonas aeruginosa / drug effects
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Sequence Homology, Amino Acid
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Staphylococcus aureus / drug effects
Substances
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Anti-Bacterial Agents
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Peptides