Purification of a novel antibacterial short peptide in earthworm Eisenia foetida

Acta Biochim Biophys Sin (Shanghai). 2004 Apr;36(4):297-302. doi: 10.1093/abbs/36.4.297.

Abstract

A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques. The purified peptide was applied to the MALDI-TOP MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121, was obtained, with the molecular mass of 510.8 Da and the sequence being "ACSAG".

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification*
  • Anti-Bacterial Agents / pharmacology
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Escherichia coli / drug effects
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Molecular Weight
  • Oligochaeta / chemistry*
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Peptides / pharmacology
  • Pseudomonas aeruginosa / drug effects
  • Sequence Homology, Amino Acid
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Staphylococcus aureus / drug effects

Substances

  • Anti-Bacterial Agents
  • Peptides