Extracellular nucleic acids in cultures of long-term cultivated eukaryotic cells

Ann N Y Acad Sci. 2004 Jun:1022:244-9. doi: 10.1196/annals.1318.038.

Abstract

Investigation of the kinetics of nucleic acid release by HeLa (human cervical carcinoma cell line) and A431 (human squamous carcinoma cell line) cells is presented. The released DNA and RNA were shown to accumulate in culture medium and at the cell surface. A portion of cell surface bound RNA can be eluted with PBS/EDTA. Mild trypsin treatment is required for complete detachment of cell surface bound RNA and cell surface bound DNA. Electrophoretic analysis reveals characteristic patterns of cell-associated and free RNA and DNA molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cells, Cultured
  • Chelating Agents / pharmacology
  • Culture Media / analysis
  • DNA / analysis
  • Edetic Acid / pharmacology
  • Electrophoresis, Agar Gel
  • Epithelial Cells / drug effects
  • Eukaryotic Cells / chemistry*
  • Eukaryotic Cells / drug effects
  • HeLa Cells
  • Humans
  • Kinetics
  • Nucleic Acids / analysis
  • Nucleic Acids / chemistry*
  • Nucleic Acids / drug effects
  • Nucleic Acids / metabolism*
  • RNA / analysis
  • Time Factors
  • Trypsin / pharmacology

Substances

  • Chelating Agents
  • Culture Media
  • Nucleic Acids
  • RNA
  • DNA
  • Edetic Acid
  • Trypsin