Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+

J Am Chem Soc. 2004 Jul 21;126(28):8835-41. doi: 10.1021/ja048615+.

Abstract

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)2(PVP)10]2+ [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os(II) sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)2(PVP)10]2+, and [Os(bpy)2(PVP)10]2+ had Os(II) sites that produced ECL specific for oxidized DNA, and Ru(II) sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biosensing Techniques / methods
  • DNA / analysis*
  • DNA / chemistry*
  • DNA / drug effects
  • Electrochemistry*
  • Epoxy Compounds / pharmacology
  • Luminescent Measurements
  • Oligonucleotides / analysis
  • Oligonucleotides / chemistry
  • Organometallic Compounds / chemistry*
  • Oxidation-Reduction
  • Polyvinyls / chemistry*

Substances

  • Epoxy Compounds
  • Oligonucleotides
  • Organometallic Compounds
  • Polyvinyls
  • bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))osmium(II)
  • bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))ruthenium(II)
  • DNA
  • styrene oxide