Abstract
Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)2(PVP)10]2+ [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os(II) sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)2(PVP)10]2+, and [Os(bpy)2(PVP)10]2+ had Os(II) sites that produced ECL specific for oxidized DNA, and Ru(II) sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biosensing Techniques / methods
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DNA / analysis*
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DNA / chemistry*
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DNA / drug effects
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Electrochemistry*
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Epoxy Compounds / pharmacology
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Luminescent Measurements
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Oligonucleotides / analysis
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Oligonucleotides / chemistry
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Organometallic Compounds / chemistry*
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Oxidation-Reduction
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Polyvinyls / chemistry*
Substances
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Epoxy Compounds
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Oligonucleotides
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Organometallic Compounds
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Polyvinyls
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bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))osmium(II)
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bis(2,2'-bipyridine)decakis(poly(vinyl pyridine))ruthenium(II)
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DNA
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styrene oxide