Basic fibroblast growth factor gene transfection to enhance the repair of avascular necrosis of the femoral head

Chin Med Sci J. 2004 Jun;19(2):111-5.

Abstract

Objective: To explore a new method for the therapy of avascular necrosis of the femoral head.

Method: The recombinant plasmid pCD-rbFGF was mixed with collagen and was implanted in the necrotic femoral head. Expression of basic fibroblast growth factor (bFGF) was examined by RT-PCR and immunohistochemical method. Repair of the femoral head was observed by histological and histomorphometric analysis.

Result: Expression of bFGF was detected in the femoral head transfected with bFGF gene, indicating significant increase of angiogenesis 2 weeks after gene transfection and increased new bone formation 8 weeks after gene transfection on histomorphometric analysis (P< 0.01).

Conclusion: Transfection of bFGF gene enhances bone tissue angiogenesis. Repair in osteonecrosis would be accelerated accordingly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / therapeutic use
  • Femur Head / metabolism*
  • Femur Head Necrosis / therapy*
  • Fibroblast Growth Factor 2 / biosynthesis
  • Fibroblast Growth Factor 2 / genetics*
  • Gene Expression
  • Genetic Therapy*
  • Neovascularization, Physiologic
  • Osteogenesis
  • Plasmids
  • Rabbits
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Transfection*

Substances

  • Recombinant Proteins
  • Fibroblast Growth Factor 2
  • Collagen