Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata using high-speed counter-current chromatography

Qizhen Du; Ping Chen; Gerold Jerz; Peter Winterhalter

doi:10.1016/j.chroma.2004.03.062

Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata using high-speed counter-current chromatography

J Chromatogr A. 2004 Jun 18;1040(1):147-9. doi: 10.1016/j.chroma.2004.03.062.

Authors

Qizhen Du¹, Ping Chen, Gerold Jerz, Peter Winterhalter

Affiliation

¹ Institute of Food and Biological Engineering, Hangzhou University of Commerce, Hangzhou 310035, China. qizhendu@mail.hzic.edu.cn

PMID: 15248435
DOI: 10.1016/j.chroma.2004.03.062

Abstract

Preparative separation of flavonoid glycosides in leaves extract of Ampelopsis grossedentata was conducted using high-speed counter-current chromatograph (HSCCC) with a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:6:1.5:7.5, v/v). In a single operation, 28 mg of 5,7-dihydroxy-3',4'-trihydroxyflavone-3-O-6''-rhamnose and 18 mg of 5,7-dihydroxy-3',4'-dihydroxyflavone-3-O-6''-rhamnose was obtained from 150 mg of the extract. The chemical structure of the two compounds was elucidated by electrospray ionization (EIS) MS and NMR.

MeSH terms

Ampelopsis / chemistry*
Countercurrent Distribution / methods*
Flavonoids / isolation & purification*
Glycosides / isolation & purification*
Plant Extracts / chemistry*
Spectrometry, Mass, Electrospray Ionization

Substances

Flavonoids
Glycosides
Plant Extracts